ARG43037

anti-Histone H4 acetyl (Lys91) antibody

anti-Histone H4 acetyl (Lys91) antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Histone H4 acetyl (Lys91)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Histone H4
Antigen Species Human
Immunogen Synthetic acetylated peptide around Lys91 of Human Histone H4.
Conjugation Un-conjugated
Alternate Names H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; HIST2H4; HIST2H4A

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control NIH/3T3 + TSA

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8370 Human HIST2H4A

Swiss-port # P62805 Human Histone H4

Gene Symbol H4C14
Gene Full Name H4 clustered histone 14
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Aug 2015]
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [UniProt]
Cellular Localization Nucleus. Chromosome. [UniProt]
Calculated MW 11 kDa

Images (2) Click the Picture to Zoom In

  • ARG43037 anti-Histone H4 acetyl (Lys91) antibody ICC/IF image

    Immunofluorescence: HeLa cells were treated by TSA (1 µM) at 37°C for 18 hours. Cells were stained with ARG43037 anti-Histone H4 acetyl (Lys91) antibody at 1:100 dilution.

  • ARG43037 anti-Histone H4 acetyl (Lys91) antibody WB image

    Western blot: NIH/3T3 cells were untreated (-) or treated (+) by TSA (1 µM) at 37°C for 18 hours. 25 µg of cell lysates stained with ARG43037 anti-Histone H4 acetyl (Lys91) antibody at 1:1000 dilution.