ARG45042
anti-Histone H3 (K27M mutation) antibody [RRM192]
anti-Histone H3 (K27M mutation) antibody [RRM192] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit monoclonal [RM192] recognizes Histone H3 (K27M mutation). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, WB |
| Specificity | This antibody reacts to the Histone H3 K27M mutant. No cross reactivity with wild type Histone H3. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | RM192 |
| Isotype | IgG |
| Target Name | Histone H3 |
| Immunogen | A peptide corresponding to Histone H3 K27M mutant |
| Conjugation | Un-conjugated |
| Alternate Names | H3-3A; H3.3 Histone A; H3.3A; H3F3A; H3F3; H3 Histone Family Member 3A; H3 Histone, Family 3A; Histone H3.3; BRYLIB1; H3-3B; H3.3B; H3F3B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide |
| Preservative | 0.09% sodium azide |
| Stabilizer | 50% Glycerol, 1% BSA and 0.09% |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | H3-3A |
| Gene Full Name | H3.3 Histone A |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008] |
| Function | Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [Uniprot] |
| Cellular Localization | Chromosome, Nucleosome core, Nucleus. [Uniprot] |
