ARG45027
anti-Histone H2B acetyl (Lys5) antibody [RM455]
anti-Histone H2B acetyl (Lys5) antibody [RM455] for Western blot,ELISA and Human
Overview
| Product Description | Rabbit monoclonal antibody recognizes Histone H2B acetyl (Lys5). |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Specificity | This antibody reacts to Histone H2B acetylated at Lysine 5 (K5ac). No cross reactivity with other acetylated Lysines in histones. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | RM455 |
| Isotype | IgG |
| Target Name | Histone H2B |
| Antigen Species | Human |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys5). |
| Conjugation | Un-conjugated |
| Alternate Names | H2BC3; H2B Clustered Histone 3; H2B/F; HIST1H2BB; H2BFF; Histone Cluster 1 H2B Family Member B; H2B Histone Family, Member F; Histone Cluster 1, H2bb; Histone H2B Type 1-B; Histone 1, H2bb; Histone H2B.1; Histone H2B.F; H2B-Clustered Histone 3; H2B.1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide |
| Preservative | 0.09% sodium azide |
| Stabilizer | 50% Glycerol, 1% BSA and 0.09% |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | H2BC3 |
| Gene Full Name | H2B Clustered Histone 3 |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [Uniprot] |
| Cellular Localization | Chromosome, Nucleosome core, Nucleus. [Uniprot] |
