ARG57250
anti-Histone H2B acetyl (Lys23) antibody [RM260]
anti-Histone H2B acetyl (Lys23) antibody [RM260] for ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Monoclonal antibody [RM260] recognizes Histone H2B acetyl (Lys23) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | RM260 |
| Isotype | IgG |
| Target Name | Histone H2B |
| Antigen Species | Others |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys23). |
| Conjugation | Un-conjugated |
| Alternate Names | Histone H2B.f; Histone H2B type 1-B; Histone H2B.1; H2B/f; H2BFF; H2B.1 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide, 50% Glycerol and 1% BSA. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol and 1% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HIST1H2BB |
| Gene Full Name | histone cluster 1, H2bb |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [UniProt] |
| PTM | Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity). Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. |
Images (3) Click the Picture to Zoom In
-
ARG57250 anti-Histone H2B acetyl (Lys23) antibody [RM260] ICC/IF image
Immunofluorescence: HeLa cells treated with sodium butyrate, stained with ARG57250 anti-Histone H2B acetyl (Lys23) antibody [RM260] (red). Actin filaments have been labeled with fluorescein phalloidin (green).
-
ARG57250 anti-Histone H2B acetyl (Lys23) antibody [RM260] WB image
Western blot: Acid extracts of HeLa cells 1) treated or 2) untreated with sodium butyrate, and 3) Recombinant Histone H2B, stained with ARG57250 anti-Histone H2B acetyl (Lys23) antibody [RM260] at 0.5 µg/ml.
-
ARG57250 anti-Histone H2B acetyl (Lys23) antibody [RM260] Specificity test image
ARG57250 anti-Histone H2B acetyl (Lys23) antibody [RM260] specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12 (K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or non-modified Lysine 23 in histone H2B.
