ARG56938
anti-Hexokinase (pan) antibody [4D7]
anti-Hexokinase (pan) antibody [4D7] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [4D7] recognizes Hexokinase (pan) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4D7 |
| Isotype | IgG2a, kappa |
| Target Name | Hexokinase (pan) |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-917 of Human Hexokinase (pan). |
| Conjugation | Un-conjugated |
| Alternate Names | Brain form hexokinase; HK1-tc; HK1-tb; HK1-ta; HMSNR; Hexokinase-1; Hexokinase type I; HK I; HKI; HXK1; EC 2.7.1.1; HKD |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HK1 |
| Gene Full Name | hexokinase 1 |
| Background | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009] |
| Calculated MW | 102 kDa |
Images (4) Click the Picture to Zoom In
-
ARG56938 anti-Hexokinase (pan) antibody [4D7] ICC/IF image
Immunoflorescense: HeLa cells stained with ARG56938 anti-Hexokinase (pan) antibody [4D7] at 1:1000 (Green).
Hoechst 33342 (Blue) for nucleus staining.
-
ARG56938 anti-Hexokinase (pan) antibody [4D7] WB image
Western blot: 20 ng of 1) Hexokinase 1 recombinant protein, 2) Hexokinase 2 recombinant protein, 3) Hexokinase 3 recombinant protein, 4) Hexokinase 4 recombinant protein stained with ARG56938 anti-Hexokinase (pan) antibody [4D7] at 1:1000.
-
ARG56938 anti-Hexokinase (pan) antibody [4D7] WB image
Western blot: 40 µg of 1) HepG2 cell lysate, 2) HeLa cell lysate, 3) Jurkat cell lysate, 4) K562 cell lysate stained with ARG56938 anti-Hexokinase (pan) antibody [4D7] at 1:1000.
-
ARG56938 anti-Hexokinase (pan) antibody [4D7] WB image
Western blot: 40 µg of Brain tissue lysate stained with ARG56938 anti-Hexokinase (pan) antibody [4D7] at 1:1000.
