ARG43459

anti-Hexokinase 1 antibody

anti-Hexokinase 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Hexokinase 1.
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Hexokinase 1
Antigen Species Human
Immunogen Purified recombinant protein corresponding to human Hexokinase 1.
Conjugation Un-conjugated
Protein Full Name Hexokinase-1
Alternate Names HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 15275 Mouse HK1

GeneID: 25058 Rat HK1

GeneID: 3098 Human HK1

Gene Symbol HK1
Gene Full Name hexokinase 1
Background Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]

Images (6) Click the Picture to Zoom In

  • ARG43459 anti-Hexokinase 1 antibody ICC/IF image

    Immunofluorescence: A-549 stained with ARG43459 anti-Hexokinase 1 antibody at 1:100 dilution.

  • ARG43459 anti-Hexokinase 1 antibody WB image

    Western blot: MCF7 and U-87MG stained with ARG43459 anti-Hexokinase 1 antibody at 1:1000 dilution.

  • ARG43459 anti-Hexokinase 1 antibody IHC-P image

    Immunohistochemistry: Rat kidney stained with ARG43459 anti-Hexokinase 1 antibody at 1:100 dilution.

  • ARG43459 anti-Hexokinase 1 antibody WB image

    Western blot: Rat testis and Rat brain stained with ARG43459 anti-Hexokinase 1 antibody at 1:1000 dilution.

  • ARG43459 anti-Hexokinase 1 antibody IHC-P image

    Immunohistochemistry: Mouse kidney stained with ARG43459 anti-Hexokinase 1 antibody at 1:100 dilution.

  • ARG43459 anti-Hexokinase 1 antibody WB image

    Western blot: Mouse testis, Mouse brain and Mouse heart stained with ARG43459 anti-Hexokinase 1 antibody at 1:1000 dilution.