ARG56947
anti-Hexokinase 1/2 antibody [1A7]
anti-Hexokinase 1/2 antibody [1A7] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [1A7] recognizes Hexokinase 1/2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1A7 |
| Isotype | IgG1, kappa |
| Target Name | Hexokinase 1/2 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-917 of Human Hexokinase 1/2. |
| Conjugation | Un-conjugated |
| Alternate Names | Muscle form hexokinase; HK II; Hexokinase-2; Hexokinase type II; EC 2.7.1.1; HXK2; HKII |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HK2 |
| Gene Full Name | hexokinase 2 |
| Background | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009] |
| Calculated MW | 102 kDa |
Images (5) Click the Picture to Zoom In
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ARG56947 anti-Hexokinase 1/2 antibody [1A7] ICC/IF image
Immunoflorescense: Jurkat cell line stained with ARG56947 anti-Hexokinase 1/2 antibody [1A7] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56947 anti-Hexokinase 1/2 antibody [1A7] FACS image
Flow Cytometry: Jurkat cell line stained with ARG56947 anti-Hexokinase 1/2 antibody [1A7] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG56947 anti-Hexokinase 1/2 antibody [1A7] WB image
Western blot: 20 ng of 1) Hexokinase 1 recombinant protein, 2) Hexokinase 2 recombinant protein, 3) Hexokinase 3 recombinant protein, 4) Hexokinase 4 recombinant protein stained with ARG56947 anti-Hexokinase 1/2 antibody [1A7] at 1:1000.
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ARG56947 anti-Hexokinase 1/2 antibody [1A7] WB image
Western blot: 40 µg of K562 cell lysate stained with ARG56947 anti-Hexokinase 1/2 antibody [1A7] at 1:1000.
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ARG56947 anti-Hexokinase 1/2 antibody [1A7] WB image
Western blot: 35 µg of Jurkat cell lysate stained with ARG56947 anti-Hexokinase 1/2 antibody [1A7] at 1:2000.
