ARG59630
anti-Hemoglobin epsilon antibody
anti-Hemoglobin epsilon antibody for Flow cytometry,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Hemoglobin epsilon |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Rb |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Hemoglobin epsilon |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 55-83 of Human Hemoglobin epsilon. |
| Conjugation | Un-conjugated |
| Alternate Names | HBE; Epsilon-globin; Hemoglobin subunit epsilon; Hemoglobin epsilon chain |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Human placenta |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HBE1 |
| Gene Full Name | hemoglobin, epsilon 1 |
| Background | The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' [provided by RefSeq, Jul 2008] |
| Function | The epsilon chain is a beta-type chain of early mammalian embryonic hemoglobin. [UniProt] |
| Calculated MW | 16 kDa |
Images (2) Click the Picture to Zoom In
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ARG59630 anti-Hemoglobin epsilon antibody WB image
Western blot: 20 µg of Human placenta lysate stained with ARG59630 anti-Hemoglobin epsilon antibody at 1:8000 dilution.
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ARG59630 anti-Hemoglobin epsilon antibody FACS image
Flow Cytometry: K562 cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG59630 anti-Hemoglobin epsilon antibody (right histogram) at 1:25, 60 min at 37°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
