ARG54195
anti-Helios antibody [22F6] (PE)
anti-Helios antibody [22F6] (PE) for Flow cytometry and Human,Mouse
Controls and Markers antibody; Gene Regulation antibody; Immune System antibody
Overview
| Product Description | PE-conjugated Hamster Monoclonal antibody [22F6] recognizes Helios |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS |
| Specificity | The armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor expressed in some hematopoietic stem cells, and at high levels in thymicderived regulatory T cells. |
| Host | Hamster |
| Clonality | Monoclonal |
| Clone | 22F6 |
| Isotype | IgG |
| Target Name | Helios |
| Immunogen | Peptide coresponding to the amino acids 51-107 of Helios |
| Conjugation | PE |
| Alternate Names | HELIOS; ZNF1A2; Ikaros family zinc finger protein 2; ANF1A2; Zinc finger protein Helios; ZNFN1A2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IKZF2 |
| Gene Full Name | IKAROS family zinc finger 2 (Helios) |
| Background | Helios, also known as IKZF2 (Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells. |
| Function | Associates with Ikaros at centromeric heterochromatin. [UniProt] |
| Research Area | Controls and Markers antibody; Gene Regulation antibody; Immune System antibody |
| Calculated MW | 60 kDa |
Images (1) Click the Picture to Zoom In
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ARG54195 anti-Helios antibody [22F6] (PE) FACS image
Flow Cytometry: Fixed and permeabilized human peripheral blood lymphocytes stained with ARG54195 anti-Helios antibody [22F6] (PE).
