ARG63296
anti-HSPC150 / UBE2T antibody
anti-HSPC150 / UBE2T antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes HSPC150 / UBE2T |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HSPC150 / UBE2T |
| Antigen Species | Human |
| Immunogen | C-QLVGIEKKFHPDV |
| Conjugation | Un-conjugated |
| Alternate Names | EC 6.3.2.19; FANCT; PIG50; Cell proliferation-inducing gene 50 protein; Ubiquitin carrier protein T; HSPC150; Ubiquitin-conjugating enzyme E2 T; Ubiquitin-protein ligase T |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM, Mar 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 23 kDa |
| PTM | Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-182 are unclear: according to a report, monoubiquitination inactivates E2 enzyme activity (PubMed:16916645). In contrast, according to another report, autoubiquitination does not affect E2 enzyme activity (PubMed:19111657). |
Images (1) Click the Picture to Zoom In
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ARG63296 anti-HSPC150 / UBE2T antibody WB image
Western Blot: K562 lysate (35 µg protein in RIPA buffer) stained with ARG63296 anti-HSPC150 / UBE2T antibody at 0.1 µg/ml dilution.
