ARG58827
anti-HSF4 antibody
anti-HSF4 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HSF4 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Bov |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HSF4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 149-185 of Human HSF4 (VQESTEARLRELRQQNEILWREVVTLRQSHGQQHRVI). |
| Conjugation | Un-conjugated |
| Alternate Names | CTRCT5; Heat shock transcription factor 4; HSF 4; Heat shock factor protein 4; HSTF 4; CTM; hHSF4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSF4 |
| Gene Full Name | heat shock transcription factor 4 |
| Background | Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described. [provided by RefSeq, Jul 2008] |
| Function | DNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 53 kDa |
| PTM | Phosphorylated mainly on serine residues. Phosphorylation on Ser-298 promotes sumoylation on Lys-293. Isoform HSF4B is constitutively sumoylated. Sumoylation represses the transcriptional activity and is promoted by phosphorylation on Ser-298. HSFA is not sumoylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG58827 anti-HSF4 antibody WB image
Western blot: 40 µg of HeLa and MCF-7 cell lysates stained with ARG58827 anti-HSF4 antibody at 0.5 µg/ml dilution.
