ARG66339
anti-HRP antibody [SQab1887]
anti-HRP antibody [SQab1887] for Flow cytometry,ICC/IF,Western blot and Other
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1887] recognizes HRP |
|---|---|
| Tested Reactivity | Other |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1887 |
| Isotype | IgG |
| Target Name | HRP |
| Antigen Species | Others |
| Immunogen | Full length native protein (purified) corresponding to HRP. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Calculated MW | 39 kDa |
|---|
Images (3) Click the Picture to Zoom In
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ARG66339 anti-HRP antibody [SQab1887] ICC/IF image
Immunofluorescence: HeLa cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66339 anti-HRP antibody [SQab1887] (green) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66339 anti-HRP antibody [SQab1887] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66339 anti-HRP antibody [SQab1887] (red) at 1:50 dilution in 1x PBS/1% BSA for 60 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66339 anti-HRP antibody [SQab1887] WB image
Western blot: 20 ng of HRP protein stained with ARG66339 anti-HRP antibody [SQab1887] at 1:10000 dilution.
