ARG65629
anti-HOXA5 antibody
anti-HOXA5 antibody for Western blot and Human,Mouse,Rat,Pig
Cancer antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes HOXA5. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Cow |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Target Name | HOXA5 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 83-92 of Human HOXA5 (C-EPRYSQPATS). |
| Conjugation | Un-conjugated |
| Alternate Names | HOX1.3; HOX1; Homeobox protein Hox-1C; HOX1C; Homeobox protein Hox-A5 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HOXA5 |
| Gene Full Name | homeobox A5 |
| Background | In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Methylation of this gene may result in the loss of its expression and, since the encoded protein upregulates the tumor suppressor p53, this protein may play an important role in tumorigenesis. [provided by RefSeq, Jul 2008] |
| Function | Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Also binds to its own promoter. Binds specifically to the motif 5'-CYYNATTA[TG]Y-3'. [UniProt] |
| Research Area | Cancer antibody; Gene Regulation antibody |
| Calculated MW | 29 kDa |
Images (3) Click the Picture to Zoom In
-
ARG65629 anti-HOXA5 antibody WB image
Western blot: 30 µg of 293T cell lysate stained with ARG65629 anti-HOXA5 antibody at 3 µg/ml dilution.
-
ARG65629 anti-HOXA5 antibody WB image
Western Blot: loading of (A) Human, (B) Mouse, and (C) Rat Kidney lysates (35 µg protein in RIPA buffer) stained with ARG65629 anti-HOXA5 antibody at 1 µg/ml dilution.
-
ARG65629 anti-HOXA5 antibody WB image
Western Blot: Pig Kidney lysate (35 µg protein in RIPA buffer) stained with ARG65629 anti-HOXA5 antibody at 1 µg/ml dilution.
Customer's Feedback
Excellent
Review for anti-HOXA5 antibody
Application：WB
Sample：1) Mouse kidney, 2) Rat kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：3 µg/ml
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
