ARG63783
anti-HNF4A antibody
anti-HNF4A antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes HNF4A |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Pig |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HNF4A |
| Antigen Species | Human |
| Immunogen | RLSKTLVDMDMADY-C |
| Conjugation | Un-conjugated |
| Alternate Names | Transcription factor HNF-4; HNF4a9; HNF4a8; Transcription factor 14; MODY; HNF4a7; HNF4alpha; TCF-14; TCF14; Nuclear receptor subfamily 2 group A member 1; MODY1; NR2A21; Hepatocyte nuclear factor 4-alpha; TCF; NR2A1; HNF4; FRTS4; HNF-4-alpha |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 53 kDa |
| PTM | Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA. Acetylation at Lys-458 lowers transcriptional activation by about two-fold. |
Images (3) Click the Picture to Zoom In
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ARG63783 anti-HNF4A antibody WB image
Western blot: 35 µg of HepG2 lysate stained with ARG63783 anti-HNF4A antibody at 0.1 µg/ml dilution.
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ARG63783 anti-HNF4A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63783 anti-HNF4A antibody at 2 µg/ml dilution followed by AP-staining.
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ARG63783 anti-HNF4A antibody WB image
Western blot: 35 µg of Mouse small intestine lysate (in RIPA buffer) stained with ARG63783 anti-HNF4A antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
