ARG55690

anti-HMGCS1 antibody

anti-HMGCS1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes HMGCS1
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Chk, Hm
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HMGCS1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 290-317 (Center) of Human HMGCS1.
Conjugation Un-conjugated
Alternate Names Hydroxymethylglutaryl-CoA synthase, cytoplasmic; EC 2.3.3.10; HMG-CoA synthase; 3-hydroxy-3-methylglutaryl coenzyme A synthase; HMGCS

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-P1:50 - 1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control U-87 MG

Properties

Form Liquid
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 208715 Mouse HMGCS1

GeneID: 29637 Rat HMGCS1

GeneID: 3157 Human HMGCS1

Gene Symbol HMGCS1
Gene Full Name 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble)
Function This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. [UniProt]
Cellular Localization Cytoplasm.
Calculated MW 57 kDa

Images (3) Click the Picture to Zoom In

  • ARG55690 anti-HMGCS1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung carcinoma stained with ARG55690 anti-HMGCS1 antibody.

  • ARG55690 anti-HMGCS1 antibody WB image

    Western blot: 35 µg of U-87 MG cell lysate stained with ARG55690 anti-HMGCS1 antibody.

  • ARG55690 anti-HMGCS1 antibody FACS image

    Flow Cytometry: HeLa cells stained with ARG55690 anti-HMGCS1 antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.