ARG57991

anti-HMGCR antibody

anti-HMGCR antibody for Immunoprecipitation,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes HMGCR
Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HMGCR
Antigen Species Human
Immunogen Synthetic peptide derived from Human HMGCR.
Conjugation Un-conjugated
Alternate Names LDLCQ3; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; EC 1.1.1.34; HMG-CoA reductase

Application Instructions

Application Suggestion
Tested Application Dilution
IP1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat
Observed Size ~ 98 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3156 Human HMGCR

Swiss-port # P04035 Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase

Gene Symbol HMGCR
Gene Full Name 3-hydroxy-3-methylglutaryl-CoA reductase
Background HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Function Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins. [UniProt]
Cellular Localization Endoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt]
Calculated MW 97 kDa
PTM N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner.

Undergoes sterol-mediated ubiquitination and ER-association degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1. This INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG57991 anti-HMGCR antibody WB image

    Western blot: Jurkat cell lysate stained with ARG57991 anti-HMGCR antibody.