ARG56393

anti-HMGCL antibody

anti-HMGCL antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes HMGCL
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HMGCL
Antigen Species Human
Immunogen Recombinant protein of Human HMGCL
Conjugation Un-conjugated
Alternate Names EC 4.1.3.4; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase; HL; Hydroxymethylglutaryl-CoA lyase, mitochondrial

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control SKOV3

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 15356 Mouse HMGCL

GeneID: 3155 Human HMGCL

GeneID: 79238 Rat HMGCL

Gene Symbol HMGCL
Gene Full Name 3-hydroxymethyl-3-methylglutaryl-CoA lyase
Background The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Function Key enzyme in ketogenesis (ketone body formation). Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism. [UniProt]
Calculated MW 34 kDa

Images (2) Click the Picture to Zoom In

  • ARG56393 anti-HMGCL antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney stained with ARG56393 anti-HMGCL antibody at 1:100 dilution.

  • ARG56393 anti-HMGCL antibody WB image

    Western blot: SKOV3 cell lysate stained with ARG56393 anti-HMGCL antibody.