ARG65035
anti-HMGA2 / HMGI-C antibody
anti-HMGA2 / HMGI-C antibody for Western blot and Human
Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes HMGA2 / HMGI-C |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Dog |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_003474.1; NP_003475.1) and is not expected to cross-react with HMGA1. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HMGA2 / HMGI-C |
| Antigen Species | Human |
| Immunogen | C-KAAQKKAEATGEK |
| Conjugation | Un-conjugated |
| Alternate Names | HMGIC; BABL; LIPO; HMGI-C; High mobility group AT-hook protein 2; STQTL9; High mobility group protein HMGI-C |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P52926 Human High mobility group protein HMGI-C |
|---|---|
| Background | This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 12 kDa |
| PTM | Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. Phosphorylated by NEK2 (By similarity). |
Images (1) Click the Picture to Zoom In
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ARG65035 anti-HMGA2 / HMGI-C antibody WB image
Western blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG65035 anti-HMGA2 / HMGI-C antibody at 1 µg/ml dilution.
