ARG10093
anti-HLA G antibody [MEM-G/9] (Azide free)
anti-HLA G antibody [MEM-G/9] (Azide free) for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human
Immune System antibody
Overview
| Product Description | Azide free Mouse Monoclonal antibody [MEM-G/9] recognizes HLA G |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms |
| Tested Application | ELISA, FACS, ICC/IF, IHC-Fr, IP |
| Specificity | The clone MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-G/9 |
| Isotype | IgG1 |
| Target Name | HLA G |
| Antigen Species | Human |
| Immunogen | Recombinant human HLA-G refolded with beta2-microglobulin and peptide. |
| Conjugation | Un-conjugated |
| Alternate Names | HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G |
Application Instructions
| Application Suggestion |
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| Application Note | ELISA: Application note: The clone MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with clone B2M-01 or with clone W6/32. Coating antibody (10 μg/ml); Detection antibody (biotin or peroxidase conjugate; 1 μg/ml) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | FACS: JEG-3 Human choriocarcinoma cell line |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P17693 Human HLA class I histocompatibility antigen, alpha chain G |
|---|---|
| Gene Symbol | HLA-G |
| Gene Full Name | major histocompatibility complex, class I, G |
| Background | HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| Function | Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 38 kDa |
Images (1) Click the Picture to Zoom In
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ARG10093 anti-HLA G antibody [MEM-G/9] (Azide free) ICC/IF image
Immunofluorescence: HLA-G1 transfectants stained with ARG10093 anti-HLA G antibody [MEM-G/9] (Azide free) (green).
