ARG10099

anti-HLA G antibody [MEM-G/11] (FITC)

anti-HLA G antibody [MEM-G/11] (FITC) for Flow cytometry and Human

Immune System antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [MEM-G/11] recognizes HLA G
Tested Reactivity Hu
Tested Application FACS
Specificity The clone MEM-G/11 recognizes HLA-G1 antigen. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Host Mouse
Clonality Monoclonal
Clone MEM-G/11
Isotype IgG1
Target Name HLA G
Antigen Species Human
Immunogen Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
Conjugation FITC
Alternate Names HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3135 Human HLA-G

Swiss-port # P17693 Human HLA class I histocompatibility antigen, alpha chain G

Gene Symbol HLA-G
Gene Full Name major histocompatibility complex, class I, G
Background HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Function Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt]
Research Area Immune System antibody
Calculated MW 38 kDa

Images (1) Click the Picture to Zoom In

  • ARG10099 anti-HLA G antibody [MEM-G/11] (FITC) FACS image

    Flow Cytometry: JEG-3 Human choriocarcinoma cell stained with ARG10099 anti-HLA G antibody [MEM-G/11] (FITC).
    Total viable cells were used for analysis.