ARG10111
anti-HLA G antibody [G233]
anti-HLA G antibody [G233] for ELISA,Flow cytometry,Immunoprecipitation and Human
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [G233] recognizes HLA G |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, IP |
| Specificity | The clone G233 recognizes several isoforms of HLA-G expressed in all populations of extravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast, placental bed giant cells). HLA-G belongs to the nonclassical MHC Class I molecules (MHC Class Ib). G233 has been found not to cross-react with any other MHC Class I antigens (HLA-A, -B, -C, -E, -F). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | G233 |
| Isotype | IgG2a |
| Target Name | HLA G |
| Antigen Species | Human |
| Immunogen | HLA-A2.1/human beta2-microglobulin double transgenic mice were immunized with murine L cells transfected with both human beta2-microglobulin and HLA-G. |
| Conjugation | Un-conjugated |
| Alternate Names | HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P17693 Human HLA class I histocompatibility antigen, alpha chain G |
|---|---|
| Gene Symbol | HLA-G |
| Gene Full Name | major histocompatibility complex, class I, G |
| Background | HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| Function | Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt] |
| Research Area | Immune System antibody |
| Calculated MW | 38 kDa |
Images (2) Click the Picture to Zoom In
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ARG10111 anti-HLA G antibody [G233] IP image
Immunoprecipitation: HLA-G from HLA-G1 transfectants (LCL-HLA-G1) immunoprecipitated by ARG10106 anti-HLA G antibody [MEM-G/9] and protein G. HLA-G was stained with ARG10111 anti-HLA G antibody [G233] in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
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ARG10111 anti-HLA G antibody [G233] FACS image
Flow Cytometry: HLA-G transfectants stained with ARG10111 anti-HLA G antibody [G233], followed by APC-conjugated Goat anti-Mouse antibody.
Clone References
The C-terminal amino acid of the MHC-I heavy chain is critical for binding to Derlin-1 in human cytomegalovirus US11-induced MHC-I degradation.
Identification of circulating nonclassic human leukocyte antigen G (HLA-G)-like molecules in exudates.
Retrieval of trophoblast cells from the cervical canal for prediction of abnormal pregnancy: a pilot study.
Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment.
