ARG41727
anti-HLA DMB antibody
anti-HLA DMB antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HLA DMB |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HLA DMB |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 81-100 of Human HLA DMB. (NQKDTLMQRLRNGLQNCATH) |
| Conjugation | Un-conjugated |
| Alternate Names | RING7; D6S221E; HLA class II histocompatibility antigen, DM beta chain; MHC class II antigen DMB; Really interesting new gene 7 protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | ~ 29 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P28068 Human HLA class II histocompatibility antigen, DM beta chain |
|---|---|
| Gene Symbol | HLA-DMB |
| Gene Full Name | major histocompatibility complex, class II, DM beta |
| Background | HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| Function | Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. [UniProt] |
| Cellular Localization | Late endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=Localizes to late endocytic compartment. Associates with lysosome membranes. [UniProt] |
| Calculated MW | 29 kDa |
Images (2) Click the Picture to Zoom In
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ARG41727 anti-HLA DMB antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue stained with ARG41727 anti-HLA DMB antibody.
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ARG41727 anti-HLA DMB antibody WB image
Western blot: 40 µg of Jurkat, Raji and HuT whole cell lysates stained with ARG41727 anti-HLA DMB antibody at 0.5 µg/ml dilution.
