ARG63002
anti-HIV protease antibody [1696]
anti-HIV protease antibody [1696] for ELISA,Functional study,Western blot and HIV
Immune System antibody; Microbiology and Infectious Disease antibody
Overview
| Product Description | Mouse Monoclonal antibody [1696] recognizes HIV protease |
|---|---|
| Tested Reactivity | HIV |
| Tested Application | ELISA, FuncSt, WB |
| Specificity | The clone 1696 recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2), an enzyme that hydrolyzes polyproteins of HIV viruses into functional proteins. 1696 does not react with the precursor. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1696 |
| Isotype | IgG1 |
| Target Name | HIV protease |
| Antigen Species | HIV |
| Immunogen | Bacterially expressed full-length HIV-1 protease |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: The clone 1696 strongly inhibits the enzyme activity of HIV-1 and HIV-2 proteases. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | The HIV protease (PR) hydrolyzes polyproteins of HIV virus into functional protein products that are essential for its assembly and subsequent activity. This maturation process occurs as the virion buds from the host cell. HIV protease inhibitors are used in the treatment of patients with AIDS and were considered the first breakthrough in over a decade of AIDS research. HIV protease inhibitors can lower the viral load carried by AIDS patients. |
|---|---|
| Research Area | Immune System antibody; Microbiology and Infectious Disease antibody |
Images (1) Click the Picture to Zoom In
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ARG63002 anti-HIV protease antibody [1696] Dot Blot image
Dot Blot: 1. Recombinant HIV protease (0.2 µg/ml) 2. Recombinant HIV protease (1.0 µg/ml) 3. Recombinant HIV protease (2.0 µg/ml) stained with ARG63002 anti-HIV protease antibody [1696].
Clone References
Diversification and specialization of HIV protease function during in vitro evolution.
WB /
Inhibition of the HIV-1 and HIV-2 proteases by a monoclonal antibody.
