ARG62511
anti-HGF antibody [4C12.1]
anti-HGF antibody [4C12.1] for ELISA and Human
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [4C12.1] recognizes HGF |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4C12.1 |
| Isotype | IgG2b |
| Target Name | HGF |
| Antigen Species | Human |
| Immunogen | Recombinant human HGF protein. |
| Conjugation | Un-conjugated |
| Alternate Names | HPTA; Scatter factor; F-TCF; Hepatocyte growth factor; DFNB39; Hepatopoietin-A; HGFB; SF |
Application Instructions
| Application Note | ELISA: 5 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purified |
| Buffer | 1X PBS buffer with <0.1% sodium azide. |
| Preservative | <0.1% sodium azide. |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HGF |
| Gene Full Name | hepatocyte growth factor (hepapoietin A; scatter factor) |
| Background | Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. [UniProt] |
| Cellular Localization | Cytoplasmic |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 83 kDa |
Clone References
In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor.
WB / Dog
