ARG64108

anti-HEXIM1 antibody

anti-HEXIM1 antibody for ICC/IF,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes HEXIM1
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name HEXIM1
Antigen Species Human
Immunogen C-HRQQERAPLSKFGD
Conjugation Un-conjugated
Alternate Names Protein HEXIM1; Hexamethylene bis-acetamide-inducible protein 1; EDG1; CLP1; Estrogen down-regulated gene 1 protein; Cardiac lineage protein 1; Menage a quatre protein 1; HIS1; MAQ1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF10 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10614 Human HEXIM1

GeneID: 192231 Mouse HEXIM1

Swiss-port # O94992 Human Protein HEXIM1

Swiss-port # Q8R409 Mouse Protein HEXIM1

Background Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 41 kDa

Images (3) Click the Picture to Zoom In

  • ARG64108 anti-HEXIM1 antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG64108 anti-HEXIM1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG64108 anti-HEXIM1 antibody WB image

    Western blot: 35 µg of Human (A) and Mouse (B) heart lysates (in RIPA buffer) stained with ARG64108 anti-HEXIM1 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.

  • ARG64108 anti-HEXIM1 antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG64108 anti-HEXIM1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.