ARG44503

anti-HENMT1 antibody

anti-HENMT1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes HENMT1
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HENMT1
Antigen Species Human
Immunogen Human HENMT1 recombinant protein
Conjugation Un-conjugated
Alternate Names HENMT1; HEN Methyltransferase 1; HEN1 Methyltransferase Homolog 1; C1orf59; HEN1; Small RNA 2'-O-Methyltransferase; FLJ30525; HEN1 Methyltransferase Homolog 1 (Arabidopsis); Hua Enhancer 1 Homolog 1 (Arabidopsis); Chromosome 1 Open Reading Frame 59; Hua Enhancer 1 Homolog 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified with Immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 113802 Human HENMT1

GeneID: 66715 Mouse HENMT1

Swiss-port # Q5T8I9 Human Small RNA 2'-O-methyltransferase

Swiss-port # Q8CAE2 Mouse Small RNA 2'-O-methyltransferase

Gene Symbol HENMT1
Gene Full Name HEN Methyltransferase 1
Background Enables small RNA 2'-O-methyltransferase. Involved in RNA methylation. Predicted to be located in P granule. Predicted to be active in cytoplasm and nucleus.
Function Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.
Cellular Localization Cytoplasm
Calculated MW 45 kDa

Images (4) Click the Picture to Zoom In

  • ARG44503 anti-HENMT1 antibody ICC/IF image

    Immunofluorescence: HeLa stained with ARG44503 anti-HENMT1 antibody at 5 μg/mL dilution.

  • ARG44503 anti-HENMT1 antibody WB image

    Western blot: Jurkat, MCF-7 and HEL stained with ARG44503 anti-HENMT1 antibody at 0.5 μg/mL dilution.

  • ARG44503 anti-HENMT1 antibody FACS image

    Flow Cytometry: JK stained with ARG44503 anti-HENMT1 antibody at 1 μg/10^6 cells dilution.

  • ARG44503 anti-HENMT1 antibody WB image

    Western blot: RAW264.7 stained with ARG44503 anti-HENMT1 antibody at 0.5 μg/mL dilution.