Overview

Product Description	Rabbit Polyclonal antibody recognizes HDAC8
Tested Reactivity	Hu, Ms, Rat
Tested Application	FACS, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	HDAC8
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human HDAC8.
Conjugation	Un-conjugated
Alternate Names	MRXS6; WTS; CDLS5; Histone deacetylase 8; HD8; EC 3.5.1.98; CDA07; RPD3; HDACL1

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:50 IP 1:50 WB 1:5000 - 1:20000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 363481 Rat HDAC8 GeneID: 55869 Human HDAC8 GeneID: 70315 Mouse HDAC8 Swiss-port # B1WC68 Rat Histone deacetylase 8 Swiss-port # Q8VH37 Mouse Histone deacetylase 8 Swiss-port # Q9BY41 Human Histone deacetylase 8 More
Gene Symbol	HDAC8
Gene Full Name	histone deacetylase 8
Background	Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Function	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility. [UniProt]
Cellular Localization	Nucleus. Cytoplasm. Note=Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. [UniProt]
Calculated MW	42 kDa
PTM	Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. [UniProt]

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