ARG20183
anti-HDAC6 antibody
anti-HDAC6 antibody for Immunohistochemistry,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HDAC6 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC, WB |
| Specificity | The antibody detects ~134 kDa HDAC-6. The antibody does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HDAC6 |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide mapping to the N-terminus of mouse HDAC-6 |
| Conjugation | Un-conjugated |
| Alternate Names | HD6; EC 3.5.1.98; PPP1R90; CPBHM; JM21; Histone deacetylase 6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | |Jurkat cell lysate can be used as a positive control. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified Antibody |
| Buffer | PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal |
| Preservative | 0.01% Thimerosal |
| Stabilizer | 30% Glycerol, 0.5% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HDAC6 |
| Gene Full Name | histone deacetylase 6 |
| Background | Human HDAC6 (1215 a.a. residues) possesses two separate putative catalytic domains, both of which are fully functional and contribute independently to the overall activity of HDAC6. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. HDAC6 may participate in coordinating expression of a group of genes involved in the remoldeling of chromatin during cell differentiation. |
| Calculated MW | 131 kDa |
Images (1) Click the Picture to Zoom In
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ARG20183 anti-HDAC6 antibody WB image
Western Blot: Jurkat cell lysate stained with anti-HDAC6 antibody (ARG20183).
The antibody detects ~134 kDa HDAC-6. Jurkat cell lysate can be used as a positive control. The antibody does not cross-react with other HDAC proteins includi ng HDAC1, 2, 3, 4, 5, 7, and 8.
