ARG10064
anti-HCV Core antigen / HCcAg antibody [B2]
anti-HCV Core antigen / HCcAg antibody [B2] for ELISA,ICC/IF,Western blot and HCV
Microbiology and Infectious Disease antibody
Overview
| Product Description | Mouse Monoclonal antibody [B2] recognizes Hepatitis C virus core antigen |
|---|---|
| Tested Reactivity | HCV |
| Tested Application | ELISA, ICC/IF, WB |
| Specificity | No cross reaction with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B2 |
| Isotype | IgG1, kappa |
| Target Name | HCV Core antigen / HCcAg |
| Antigen Species | HCV |
| Immunogen | Synthetic peptides derived from HCV core protein |
| Conjugation | Un-conjugated |
Application Instructions
| Application Note | ELISA: The mAb reacts with human hepatitis C virus core protein, and doesn’t react with HCV non-structure protein. Western Blot: The mAb, when used at concentration of 0.1-0.5μg/mL, will allow visualization of 0.1 μg/lane of recombinant core protein C + envelope protein M, 0.5μg /lane synthetic core protein C, and 0.1μg /lane recombinant chimeric HCV polyprotein (552 aa, around 60 kDa). The mAb works on blots transferred from both reducing and non-reducing PAGE gel. The mAb recognizes in-vitro translated HCV core protein (around 20 kDa) when used at concentration of 1 μg/mL. Immunofluorescent analysis: The mAb recognizes HCV core in HCV core expressing plasmid transfected cells at 1:200 dilution. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | 0.01M PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of Core Protein (Capsid Protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B. |
| Research Area | Microbiology and Infectious Disease antibody |
Images (3) Click the Picture to Zoom In
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ARG10064 anti-HCV Core antigen / HCcAg antibody [B2] ICC/IF image
Immunofluorescence: Cells stained with ARG10064 anti-HCV Core antigen / HCcAg antibody [B2] (green).
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ARG10064 anti-HCV Core antigen / HCcAg antibody [B2] WB image
Western blot: HCV Core protein expressing plasmid transfected 293 cells stained with ARG10064 anti-HCV Core antigen / HCcAg antibody [B2].
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ARG10064 anti-HCV Core antigen / HCcAg antibody [B2] WB image
Western blot: HCV Core protein stable expressed Huh7 cells stained with ARG10064 anti-HCV Core antigen / HCcAg antibody [B2] and anti-beta actin antibody.
