ARG10063

anti-HCV Core antigen / HCcAg antibody [A1/3D1]

Datasheet

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100 μg

389.00

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Datasheet

Overview

Product Description	Mouse Monoclonal antibody [A1/3D1] recognizes Hepatitis C virus core antigen
Tested Reactivity	HCV
Tested Application	ELISA
Specificity	No cross reaction with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).
Host	Mouse
Clonality	Monoclonal
Clone	A1/3D1
Isotype	IgG1, kappa
Target Name	HCV Core antigen / HCcAg
Antigen Species	HCV
Immunogen	Synthetic peptides derived from HCV core protein
Conjugation	Un-conjugated

Application Instructions

Application Note	ELISA: mAb A1/3D1 was tested using indirect ELISA method. The testing plates were coated with HCV non-structural protein (NS), core protein (C) and mixture of NS and C respectively. The neat culture supernatant of hybridoma showed a strong reactivity with HCV capsid protein (OD492 value > 2.0) and with mixture of NS and C (OD492 value >1.5), and showed no reactivity with HCV non-structural protein (OD492 value < 0.03). mAb A1/3D1 recognizes in-vitro translated HCV core protein. Not suitable for use in Western Blot * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Application Note

ELISA: mAb A1/3D1 was tested using indirect ELISA method. The testing plates were coated with HCV non-structural protein (NS), core protein (C) and mixture of NS and C respectively. The neat culture supernatant of hybridoma showed a strong reactivity with HCV capsid protein (OD492 value > 2.0) and with mixture of NS and C (OD492 value >1.5), and showed no reactivity with HCV non-structural protein (OD492 value < 0.03). mAb A1/3D1 recognizes in-vitro translated HCV core protein.

Not suitable for use in Western Blot

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Protein G affinity purified
Buffer	0.01M PBS (pH 7.2)
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 951475 HCV HCVgp1
Background	Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of Core Protein (Capsid Protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.
Research Area	Microbiology and Infectious Disease antibody