ARG44496

anti-HAUS3 antibody

anti-HAUS3 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes HAUS3
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Target Name HAUS3
Antigen Species Human
Immunogen Human HAUS3 recombinant protein
Conjugation Un-conjugated
Alternate Names HAUS3; HAUS Augmin Like Complex Subunit 3; C4orf15; Dgt3; IT1; HAUS Augmin-Like Complex Subunit 3; MGC4701; Chromosome 4 Open Reading Frame 15

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 231123 Mouse HAUS3

GeneID: 79441 Human HAUS3

Swiss-port # Q68CZ6 Human HAUS augmin-like complex subunit 3

Swiss-port # Q8QZX2 Mouse HAUS augmin-like complex subunit 3

Gene Symbol HAUS3
Gene Full Name HAUS Augmin Like Complex Subunit 3
Background This gene encodes a component of the HAUS augmin-like protein complex, which plays a key role in cytokinesis and mitosis. Disruption of the encoded protein causes mitotic defects resulting from fragmentation of centrosomes and microtubule destabilization. This gene shares its 5' exons with some transcripts from overlapping GeneID: 353497, which encodes a DNA polymerase. Alternative splicing results in multiple transcript variants.
Function Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
Cellular Localization Cytoplasm, Cytoskeleton, Microtubule
Calculated MW 70 kDa
PTM Acetylation

Images (5) Click the Picture to Zoom In

  • ARG44496 anti-HAUS3 antibody ICC/IF image

    Immunofluorescence: HeLa stained with ARG44496 anti-HAUS3 antibody at 5 μg/mL dilution.

  • ARG44496 anti-HAUS3 antibody WB image

    Western blot: HepG2 and HeLa stained with ARG44496 anti-HAUS3 antibody at 0.5 μg/mL dilution.

  • ARG44496 anti-HAUS3 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG44496 anti-HAUS3 antibody at 1 μg/10^6 cells dilution.

  • ARG44496 anti-HAUS3 antibody WB image

    Western blot: Rat brain stained with ARG44496 anti-HAUS3 antibody at 0.5 μg/mL dilution.

  • ARG44496 anti-HAUS3 antibody WB image

    Western blot: Mouse brain stained with ARG44496 anti-HAUS3 antibody at 0.5 μg/mL dilution.