ARG66389
anti-HA tag antibody [SQab18117]
anti-HA tag antibody [SQab18117] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Other
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab18117] recognizes HA tag |
|---|---|
| Tested Reactivity | Other |
| Tested Application | FACS, ICC/IF, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab18117 |
| Isotype | IgG |
| Target Name | HA tag |
| Antigen Species | Others |
| Immunogen | YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (6) Click the Picture to Zoom In
-
ARG66389 anti-HA tag antibody [SQab18117] ICC/IF image
Immunofluorescence: 293 cells transfected with N-terminal HA tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:2000 at 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.
-
ARG66389 anti-HA tag antibody [SQab18117] FACS image
Flow Cytometry: 293 cells transfected with N-terminal (green) or C-terminal (red) HA tagged gene. The cells were then stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:2000 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
-
ARG66389 anti-HA tag antibody [SQab18117] WB image
Western blot: 1) 1 µg of 293 cells transfected with C-terminal HA tagged gene and stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:20000 dilution. 2) 10 µg of 293 cells transfected with N-terminal HA tagged gene and stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:1000 dilution. 3) 10 µg of 293 cells (without any transfection) stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:400 dilution.
-
ARG66389 anti-HA tag antibody [SQab18117] IP image
Immunoprecipitation: 0.2 mg of 293 whole cell lysate (transfected with N-terminal HA tagged gene) was immunoprecipitated (1:10 dilution) and stained with ARG66389 anti-HA tag antibody [SQab18117].
Lane 1: Immunoprecipitation in 293 whole cell lysate (transfected with N-terminal HA tagged gene)
Lane 2: PBS instead of Primary Ab in 293 whole cell lysate (transfected with N-terminal HA tagged gene)
Lane 3: 293 whole cell lysate (transfected with N-terminal HA tagged gene), 10 µg (input) -
ARG66389 anti-HA tag antibody [SQab18117] ICC/IF image
Immunofluorescence: 293 cells transfected with C-terminal HA tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG66389 anti-HA tag antibody [SQab18117] at 1:2000 at 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.
-
ARG66389 anti-HA tag antibody [SQab18117] IP image
Immunoprecipitation: 0.2 mg of 293 whole cell lysate (transfected with C-terminal HA tagged gene) was immunoprecipitated (1:10 dilution) and stained with ARG66389 anti-HA tag antibody [SQab18117].
Lane 1: Immunoprecipitation in 293 whole cell lysate (transfected with C-terminal HA tagged gene)
Lane 2: PBS instead of Primary Ab in 293 whole cell lysate (transfected with C-terminal HA tagged gene)
Lane 3: 293 whole cell lysate (transfected with C-terminal HA tagged gene), 10 µg (input)
Customer's Feedback
Good
Review for anti-HA tag antibody [SQab18117]
Application：IHC
Sample：Frozen Mouse brain tissue section
Sample Preparation Method：PBS and 4% PFA perfusion
Fixation Buffer：4% PFA
Fixation Time：overnight
Antigen Retrieval Buffer：Citrate pH 6.0
Permeabilization Buffer：0.3% PBST (Triton x-100)
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
