ARG62338
anti-HA tag antibody [HA.C5]
anti-HA tag antibody [HA.C5] for Dot blot,ELISA,Flow cytometry,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Other
Controls and Markers antibody; Gene Regulation antibody
Publication2
Overview
| Product Description | Mouse Monoclonal antibody [HA.C5] recognizes HA tag |
|---|---|
| Tested Reactivity | Other |
| Tested Application | Dot, ELISA, FACS, ICC/IF, IHC, IP, WB |
| Specificity | Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HA.C5 |
| Isotype | IgG3 |
| Target Name | HA tag |
| Antigen Species | Others |
| Immunogen | YPYDVPDYA (HA) synthetic peptide conjugated to KLH. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A Purified |
| Purification Note | Protein A affinity chromatography from mouse ascites fluid. |
| Buffer | 10mM PBS (pH 7.2) and 0.05% Sodium azide. |
| Preservative | 0.05% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. Duo to HA-tag is from influenza virus and not be found in nomal anamils, it is useful for the protein functional analysis when the specific antibodies for the protein-of-interest are unavailable. |
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| Highlight | Related products: HA tag antibodies; Anti-Mouse IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Gene Regulation antibody |
Images (1) Click the Picture to Zoom In
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ARG62338 anti-HA tag antibody [HA.C5] WB image
Western blot: 10 µg of 293T and 293T + myc-HA-mCherry cell lysates stained with ARG62338 anti-HA tag antibody [HA.C5] at 1:1000 dilutioin.
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Review for anti-HA tag antibody [HA.C5]
Application：WB
Sample：293T and 293T + myc-HA-mCherry
Sample Loading Amount：10 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Translation initiation factor eIF4G1 modulates assembly of the polypeptide exit tunnel region in yeast ribosome biogenesis
WB / Yeast
SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK
IP /
