ARG55232

anti-H Cadherin antibody

anti-H Cadherin antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes H Cadherin
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name H Cadherin
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 146-174 (N-terminus) of Human H Cadherin.
Conjugation Un-conjugated
Alternate Names CDHH; H-cadherin; P105; Cadherin-13; Truncated cadherin; T-cad; Heart cadherin; T-cadherin

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human brain

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1012 Human CDH13

Swiss-port # P55290 Human Cadherin-13

Gene Symbol CDH13
Gene Full Name cadherin 13
Background This gene encodes a member of the cadherin superfamily. The encoded protein is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. The protein lacks the cytoplasmic domain characteristic of other cadherins, and so is not thought to be a cell-cell adhesion glycoprotein. This protein acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. The gene is hypermethylated in many types of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2011]
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor.
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 78 kDa

Images (3) Click the Picture to Zoom In

  • ARG55232 anti-H Cadherin antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue stained with ARG55232 anti-H Cadherin antibody.

  • ARG55232 anti-H Cadherin antibody WB image

    Western blot: 35 µg of Human brain lysate stained with ARG55232 anti-H Cadherin antibody at 1:1000 dilution.

  • ARG55232 anti-H Cadherin antibody FACS image

    Flow Cytometry: HepG2 cells stained with ARG55232 anti-H Cadherin antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.