ARG10821

anti-Guanylate Cyclase beta antibody

anti-Guanylate Cyclase beta antibody for Confocal microscopy (Confocal),Dot blot,ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Pig

Overview

Product Description Rabbit Polyclonal antibody recognizes Guanylate Cyclase beta
Tested Reactivity Hu, Ms, Rat, Bov, Pig
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Guanylate Cyclase beta
Antigen Species Rat
Immunogen Synthetic peptide taken within aa. 175-225 from Rat Guanylyl Cyclase beta.
Conjugation Un-conjugated
Alternate Names GC-S-beta-1; GUC1B3; GUCSB3; Soluble guanylate cyclase small subunit; GCS-beta-1; GUCY1B1; GCS-beta-3; GUCB3; EC 4.6.1.2; Guanylate cyclase soluble subunit beta-3; GC-SB3; Guanylate cyclase soluble subunit beta-1

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:100
Dot1:10000
ELISA1:10000
ICC/IF1:100
IHC-P1:100
IP1:200
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25202 Rat GUCY1B3

GeneID: 282433 Bovine GUCY1B3

GeneID: 2983 Human GUCY1B3

Gene Symbol Gucy1b3
Gene Full Name guanylate cyclase 1, soluble, beta 3
Background This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Calculated MW 71 kDa

Images (4) Click the Picture to Zoom In

  • ARG10821 anti-Guanylate Cyclase beta antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat cerebellum tissue stained with ARG10821 anti-Guanylate Cyclase beta antibody at 1:100 dilution.

  • ARG10821 anti-Guanylate Cyclase beta antibody WB image

    Western blot: Recombinant Guanylyl Cyclase beta protein stained with ARG10821 anti-Guanylate Cyclase beta antibody at 1:500 dilution.

  • ARG10821 anti-Guanylate Cyclase beta antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat cerebellum tissue stained with ARG10821 anti-Guanylate Cyclase beta antibody at 1:100 dilution.

  • ARG10821 anti-Guanylate Cyclase beta antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat cerebellum tissue stained with ARG10821 anti-Guanylate Cyclase beta antibody at 1:100 dilution.