ARG56019
anti-Glypican 3 antibody [1G12]
anti-Glypican 3 antibody [1G12] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [1G12] recognizes Glypican 3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1G12 |
| Isotype | IgG1, kappa |
| Target Name | Glypican 3 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 511-580 of Human Glypican-3 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | SGBS1; DGSX; Glypican-3; Intestinal protein OCI-5; GTR2-2; SGB; OCI-5; SDYS; MXR7; SGBS |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HepG2 | ||||||||||
| Observed Size | ~ 65 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GPC3 |
| Gene Full Name | glypican 3 |
| Background | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009] |
| Function | Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. [UniProt] |
| Cellular Localization | Cytoplasmic |
| Calculated MW | 66 kDa |
Images (4) Click the Picture to Zoom In
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ARG56019 anti-Glypican 3 antibody [1G12] ICC/IF image
Immunofluorescence: Methanol-fixed HepG2 cells stained with ARG56019 anti-Glypican 3 antibody [1G12] (green). Reddot (red) for nuclear staining.
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ARG56019 anti-Glypican 3 antibody [1G12] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human hepatocellular carcinoma stained with ARG56019 anti-Glypican 3 antibody [1G12].
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ARG56019 anti-Glypican 3 antibody [1G12] WB image
Western blot: HepG2 cell lysate stained with ARG56019 anti-Glypican 3 antibody [1G12].
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ARG56019 anti-Glypican 3 antibody [1G12] FACS image
Flow Cytometry: Methanol-fixed HepG2 cells stained with ARG56019 anti-Glypican 3 antibody [1G12] (blue); Isotype control (red).
