ARG62497
anti-Glycophorin C antibody [Ret40f]
anti-Glycophorin C antibody [Ret40f] for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Mouse Monoclonal antibody [Ret40f] recognizes Glycophorin C |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-Fr, IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Ret40f |
| Isotype | IgG1, kappa |
| Target Name | Glycophorin C |
| Immunogen | Red cell ghosts |
| Conjugation | Un-conjugated |
| Alternate Names | Glycophorin-D; CD236R; GPD; Glycophorin-C; Glycoprotein beta; Glycoconnectin; GPC; PAS-2; CD antigen CD236; GE; CD236; Sialoglycoprotein D; GYPD; PAS-2' |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat tissue section in Sodium citrate buffer (pH 6.0) or EDTA buffer (pH 8.0) IHC-Fr: Incubate for 30-60 minutes at room temperature is highly recommended. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 1X PBS buffer with <0.1% sodium azide. |
| Preservative | <0.1% sodium azide. |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GYPC |
| Gene Full Name | glycophorin C (Gerbich blood group) |
| Background | Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] |
| Function | This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells. [UniProt] |
| Cellular Localization | Cell membrane |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 14 kDa |
| PTM | O-glycosylated with core 1 or possibly core 8 glycans. |
