ARG56688

anti-Galectin 3 antibody

anti-Galectin 3 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Galectin 3
Tested Reactivity Hu
Tested Application ELISA, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Galectin 3
Antigen Species Human
Immunogen E.coli derived Recombinant Human Galectin-3.
(ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI)
Conjugation Un-conjugated
Alternate Names Laminin-binding protein; Gal-3; L-31; GALBP; Galactoside-binding protein; MAC2; GAL3; GALIG; Mac-2 antigen; CBP 35; Galectin-3; CBP35; Galactose-specific lectin 3; IgE-binding protein; L31; 35 kDa lectin; Carbohydrate-binding protein 35; Lectin L-29

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56797 as a detection antibody
IHC-P31.25 ng/ml
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3958 Human LGALS3

Swiss-port # P17931 Human Galectin-3

Gene Symbol LGALS3
Gene Full Name lectin, galactoside-binding, soluble, 3
Background This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
Function Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. [UniProt]
Calculated MW 26 kDa

Images (5) Click the Picture to Zoom In

  • ARG56688 anti-Galectin 3 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human pancreas infiltrating ductal adenocarcinoma tissue. The recommended ARG56688 anti-Galectin 3 antibody concentration is 31.25 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56688 anti-Galectin 3 antibody WB image

    Western blot: 250 - 0.24 ng of Human Galectin-3 stained with ARG56688 anti-Galectin 3 antibody, under reducing conditions.

  • ARG56688 anti-Galectin 3 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human pancreas infiltrating ductal adenocarcinoma tissue. The recommended ARG56688 anti-Galectin 3 antibody concentration is 31.25 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56688 anti-Galectin 3 antibody WB image

    Western blot: 250 - 0.24 ng of Human Galectin-3 stained with ARG56688 anti-Galectin 3 antibody, under non-reducing conditions.

  • ARG56688 anti-Galectin 3 antibody standard curve image

    Sandwich ELISA: ARG56688 anti-Galectin 3 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56797 anti-Galectin 3 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.