ARG11061
anti-Galectin 3 antibody
anti-Galectin 3 antibody for ICC/IF,Western blot and Human,Mouse,Rat,Pig
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Galectin 3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Galectin 3 |
| Antigen Species | Human |
| Immunogen | Full length recombinant Galectin 3 expressed in and purified from E. coli |
| Conjugation | Un-conjugated |
| Alternate Names | Laminin-binding protein; Gal-3; L-31; GALBP; Galactoside-binding protein; MAC2; GAL3; GALIG; Mac-2 antigen; CBP 35; Galectin-3; CBP35; Galactose-specific lectin 3; IgE-binding protein; L31; 35 kDa lectin; Carbohydrate-binding protein 35; Lectin L-29 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 30 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Unpurified |
| Buffer | Serum and 5mM Sodium azide. |
| Preservative | 5mM Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LGALS3 |
| Gene Full Name | lectin, galactoside-binding, soluble, 3 |
| Background | This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014] |
| Function | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. [UniProt] |
| Calculated MW | 26 kDa |
Images (3) Click the Picture to Zoom In
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ARG11061 anti-Galectin 3 antibody ICC/IF image
Immunofluorescence: Cortical neuron-glial cell culture from E20 Rat stained with ARG11061 anti-Galectin 3 antibody (green) at 1:2000 dilution, and costained with mouse mAb to GFAP (red) at 1:2000 dilution. The blue is Hoechst staining of nuclear DNA.
Certain glial cells express only galectin-3 protein, and appear green, while the majority of glial cells and astrocytes produces GFAP protein and so appear red, a few cells that express both protein appear orange-yellow.
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ARG11061 anti-Galectin 3 antibody ICC/IF image
Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG11061 anti-Galectin 3 antibody (green) at 1:2000 dilution and costained with ARG10719 anti-MAP2 antibody [4H5] (red) at 1:2000 dilution. Hoechst (blue) for nuclear staining.
The Galectin 3 antibody produces strong staining of certain glial cells, while MAP2 antibody labels dendrites and perikarya of mature neurons.
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ARG11061 anti-Galectin 3 antibody WB image
Western blot: Mouse heart, Mouse liver, Mouse kidney, Mouse lung, Rat cortical neuron-glial primary cell culture and Pig spinal cord lysates stained with ARG11061 anti-Galectin 3 antibody at 1:5000 dilution.
