ARG56772

anti-Galectin 1 antibody (Biotin)

anti-Galectin 1 antibody (Biotin) for ELISA,Western blot and Human

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes Galectin 1
Tested Reactivity Hu
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Galectin 1
Antigen Species Human
Immunogen E.coli derived Recombinant Human Galectin 1.
(ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD)
Conjugation Biotin
Alternate Names HBL; Gal-1; GAL1; HPL; Lectin galactoside-binding soluble 1; Galectin-1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; GBP; Beta-galactoside-binding lectin L-14-I; Putative MAPK-activating protein PM12; 14 kDa lectin; Galaptin; S-Lac lectin 1; HLBP14

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56662 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3956 Human LGALS1

Swiss-port # P09382 Human Galectin-1

Gene Symbol LGALS1
Gene Full Name lectin, galactoside-binding, soluble, 1
Background The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]
Function May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. [UniProt]
Calculated MW 15 kDa

Images (4) Click the Picture to Zoom In

  • ARG56772 anti-Galectin 1 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human Galectin-1 stained with ARG56772 anti-Galectin 1 antibody (Biotin), under reducing conditions.

  • ARG56772 anti-Galectin 1 antibody (Biotin) standard curve image

    Direct ELISA: ARG56772 anti-Galectin 1 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56772 anti-Galectin 1 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human Galectin-1 stained with ARG56772 anti-Galectin 1 antibody (Biotin), under non-reducing conditions.

  • ARG56772 anti-Galectin 1 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56772 anti-Galectin 1 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56662 anti-Galectin 1 antibody as a capture antibody. Results of a typical standard run with optical density.