ARG63784

anti-GST3 / GST pi antibody

anti-GST3 / GST pi antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes GST3 / GST pi
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name GST3 / GST pi
Antigen Species Human
Immunogen C-LADQGQSWKEEV
Conjugation Un-conjugated
Alternate Names DFN7; EC 2.5.1.18; GST3; GSTP; GSTP1-1; HEL-S-22; FAEES3; GST class-pi; PI; Glutathione S-transferase P

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.01 - 0.03 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2950 Human GSTP1

Swiss-port # P09211 Human Glutathione S-transferase P

Background Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 23 kDa

Images (3) Click the Picture to Zoom In

  • ARG63784 anti-GST3 / GST pi antibody WB image

    Western blot: 35 µg of human kidney lysate stained with ARG63784 anti-GST3 / GST pi antibody at 0.01 µg/ml dilution.

  • ARG63784 anti-GST3 / GST pi antibody WB image

    Western blot: 1) Mock transfection and 2) GSTP1 expressing plasmid transfected HEK293 cell lysate stained with ARG63784 anti-GST3 / GST pi antibody.

  • ARG63784 anti-GST3 / GST pi antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63784 anti-GST3 / GST pi antibody at 5 µg/ml dilution followed by AP-staining.