ARG66338
anti-GST tag antibody [SQab1886]
anti-GST tag antibody [SQab1886] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Other
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1886] recognizes GST tag |
|---|---|
| Tested Reactivity | Other |
| Tested Application | FACS, ICC/IF, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1886 |
| Isotype | IgG |
| Target Name | GST tag |
| Antigen Species | Others |
| Immunogen | Recombinant full length protein within aa. 1-218 of Schistosoma japonicum GST. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (6) Click the Picture to Zoom In
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ARG66338 anti-GST tag antibody [SQab1886] ICC/IF image
Immunofluorescence: 293 cells transfected with N-terminal GST tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66338 anti-GST tag antibody [SQab1886] (red) at 1:40,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66338 anti-GST tag antibody [SQab1886] FACS image
Flow Cytometry: 293 cells transfected with C-terminal (red) and N-terminal (blue) GST tagged gene. The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66338 anti-GST tag antibody [SQab1886] at 1:2,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .
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ARG66338 anti-GST tag antibody [SQab1886] WB image
Western blot: 1) 2 µg of 293 cell lysates transfected with N-terminal GST tagged gene, 2) 2 µg of 293 cell lysates transfected with C-terminal GST tagged gene, 3) 10 µg of Mock 293 cell lysates stained with ARG66338 anti-GST tag antibody [SQab1886] at 1:10000 dilution, and 4) 20 ng of two fusion proteins, one (45 kDa) with GST tag on C-terminal, the other (83 kDa) with GST tag on N-terminal stained with ARG66338 anti-GST tag antibody [SQab1886] at 1:2000 dilution.
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ARG66338 anti-GST tag antibody [SQab1886] IP image
Immunoprecipitation: 0.2 mg of 293 whole cell lysates transfected with C-terminal GST tagged gene immunoprecipitated (1:50) and stained with ARG66338 anti-GST tag antibody [SQab1886]. 1) ARG66338 IP in 293 whole cell lysates transfected with C-terminal GST tagged gene, 2) PBS instead of ARG66338 in 293 whole cell lysates transfected with C-terminal GST tagged gene, and 3) 293 whole cell lysate transfected with C-terminal GST tagged gene, 2 µg (input).
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ARG66338 anti-GST tag antibody [SQab1886] ICC/IF image
Immunofluorescence: 293 cells transfected with C-terminal GST tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66338 anti-GST tag antibody [SQab1886] (red) at 1:40,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66338 anti-GST tag antibody [SQab1886] IP image
Immunoprecipitation: 0.2 mg of 293 whole cell lysates transfected with N-terminal GST tagged gene immunoprecipitated (1:50) and stained with ARG66338 anti-GST tag antibody [SQab1886]. 1) ARG66338 IP in 293 whole cell lysates transfected with N-terminal GST tagged gene, 2) PBS instead of ARG66338 in 293 whole cell lysates transfected with N-terminal GST tagged gene, and 3) 293 whole cell lysates transfected with N-terminal GST tagged gene, 2 µg (input).
