ARG52322
anti-GSK3 beta phospho (Ser9) antibody
anti-GSK3 beta phospho (Ser9) antibody for Western blot and Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GSK3 beta phospho (Ser9) |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Bov, Chk, Dog, NHuPrm, Xenopus laevis, Zfsh |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GSK3 beta |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.7.11.26; EC 2.7.11.1; GSK-3 beta; Glycogen synthase kinase-3 beta; Serine/threonine-protein kinase GSK3B |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~46k GSK3β protein phosphorylated at Ser9 . Also weakly labels the ~51k GSK3α band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GSK3B |
| Gene Full Name | glycogen synthase kinase 3 beta |
| Background | Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3α and GSK3β. Specifically, GSK3β has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser9 phosphorylation underlies the inhibition of GSK3β by insulin (Sutherland et al., 1993). |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 47 kDa |
| PTM | Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216 (PubMed:25169422). Inactivated by phosphorylation at Ser-9 (Probable). Mono-ADP-ribosylation by PARP10 negatively regulates kinase activity. |
Images (1) Click the Picture to Zoom In
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ARG52322 anti-GSK3 beta phospho (Ser9) antibody WB image
Western blot: Rat cortex lysate showing phospho-specific immunolabeling of the ~46k GSK3p protein phosphorylated at Ser 9 stained with ARG52322 anti-GSK3 beta phospho (Ser9) antibody.
