ARG64711
anti-GRIA4 antibody
anti-GRIA4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GRIA4 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GRIA4 |
| Antigen Species | Human |
| Immunogen | C-KKLDQREYPGSETP |
| Conjugation | Un-conjugated |
| Alternate Names | GluA4; GLUR4C; GLUR4; Glutamate receptor ionotropic, AMPA 4; GluR-4; AMPA-selective glutamate receptor 4; GluR4; Glutamate receptor 4; GLURD; GluR-D |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq, Jul 2008] |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 101 kDa |
| PTM | Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity). Phosphorylated at Ser-862 by PRKCG; phosphorylation increases plasma membrane-associated GRI4 expression. |
Images (2) Click the Picture to Zoom In
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ARG64711 anti-GRIA4 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64711 anti-GRIA4 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64711 anti-GRIA4 antibody WB image
Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG64711 anti-GRIA4 antibody at 2 µg/ml dilution and incubated at RT for 1 hour.
