ARG63205
anti-GRB7 antibody
anti-GRB7 antibody for Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GRB7 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody recognises the longer isoform of this protein in A431 lysate. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GRB7 |
| Antigen Species | Human |
| Immunogen | ELDLSPPHLSSSPE-C |
| Conjugation | Un-conjugated |
| Alternate Names | Epidermal growth factor receptor GRB-7; Growth factor receptor-bound protein 7; B47; GRB7 adapter protein |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q14451 Human Growth factor receptor-bound protein 7 |
|---|---|
| Background | The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 60 kDa |
| PTM | Phosphorylated on serine and threonine residues in response to heregulin. Phosphorylated on tyrosine residues by TEK/TIE2. Phosphorylated on tyrosine residues in response to NTN1 signaling. Phosphorylation promotes stress granule disassembly during recovery after cellular stress (By similarity). Phosphorylated on tyrosine residues by PTK2/FAK1, and possibly also other kinases. Phosphorylation is enhanced by activation of receptor kinases. Tyrosine phosphorylation is essential for activation of down-stream protein kinases. |
Images (2) Click the Picture to Zoom In
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ARG63205 anti-GRB7 antibody WB image
Western Blot: A431 lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63205 anti-GRB7 antibody at 2 µg/ml dilution.
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ARG63205 anti-GRB7 antibody WB image
Western Blot: 1). Mock transfection; 2) GRB7 (RC215362) expressing plasmid transfected HEK293 cell lysate standed with ARG63205 anti-GRB7 antibody
