ARG63175
anti-GRB2 antibody
anti-GRB2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GRB2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GRB2 |
| Antigen Species | Human |
| Immunogen | C-PRNYVTPVNRNV |
| Conjugation | Un-conjugated |
| Alternate Names | SH2/SH3 adapter GRB2; EGFRBP-GRB2; Adapter protein GRB2; MST084; MSTP084; Grb3-3; ASH; Growth factor receptor-bound protein 2; NCKAP2; Protein Ash |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 25 kDa |
Images (5) Click the Picture to Zoom In
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ARG63175 anti-GRB2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lymph node tissue. Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63175 anti-GRB2 antibody at 1 µg/ml dilution followed by HRP-staining.
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ARG63175 anti-GRB2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Tonsil. (Microwaved antigen retrieval with citrate buffer pH6) stained with ARG63175 anti-GRB2 antibody at 0.3 µg/ml dilution followed by HRP-staining.
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ARG63175 anti-GRB2 antibody WB image
Western blot: 35 µg of Human thymus (A) and MOLT-4 cell (B) lysates (in RIPA buffer) stained with ARG63175 anti-GRB2 antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.
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ARG63175 anti-GRB2 antibody WB image
Western blot: 35 µg of Human (A) and Pig (B) spleen lysates (in RIPA buffer) stained with ARG63175 anti-GRB2 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
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ARG63175 anti-GRB2 antibody WB image
Western blot: 35 µg of Mouse brain (A), Rat brain (B), Mouse spleen (C) and Rat spleen (D) lysates (in RIPA buffer) stained with ARG63175 anti-GRB2 antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.
