ARG42841
anti-GRB14 antibody
anti-GRB14 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GRB14 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GRB14 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to M1-Q246 of Human GRB14. |
| Conjugation | Un-conjugated |
| Alternate Names | Growth factor receptor-bound protein 14; GRB14 adapter protein |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 61 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.01% Sodium azide and 4% Trehalose. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q14449 Human Growth factor receptor-bound protein 14 |
|---|---|
| Gene Symbol | GRB14 |
| Gene Full Name | growth factor receptor-bound protein 14 |
| Background | The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Function | Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal. [UniProt] |
| Cellular Localization | Cytoplasm. Endosome membrane; Peripheral membrane protein. Note=Upon insulin stimulation, translocates to the plasma membrane. [UniProt] |
| Calculated MW | 61 kDa |
| PTM | Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG42841 anti-GRB14 antibody ICC/IF image
Immunofluorescence: A549 cells were blocked with 10% goat serum and then stained with ARG42841 anti-GRB14 antibody (green) at 5 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42841 anti-GRB14 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human renal cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42841 anti-GRB14 antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG42841 anti-GRB14 antibody WB image
Western blot: 50 µg of sample under reducing conditions. A549 and HEK293 whole cell lysates stained with ARG42841 anti-GRB14 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42841 anti-GRB14 antibody FACS image
Flow Cytometry: U-87 MG cells were blocked with 10% normal goat serum and then stained with ARG42841 anti-GRB14 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
