ARG64038
anti-GPX4 antibody
anti-GPX4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Ferroptosis/Oxytosis Study antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes GPX4 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Pig |
| Tested Application | IHC-P, IP, WB |
| Specificity | This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GPX4 |
| Antigen Species | Human |
| Immunogen | C-EEPLVIEKDLPHY |
| Conjugation | Un-conjugated |
| Alternate Names | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; EC 1.11.1.12; MCSP; PHGPx; GSHPx-4; Glutathione peroxidase 4; GPx-4; snPHGPx; snGPx |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P36969 Human Phospholipid hydroperoxide glutathione peroxidase, mitochondrial |
|---|---|
| Background | Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. [provided by RefSeq, Jul 2008] |
| Highlight | Related products: GPX4 antibodies; GPX4 Duos / Panels; Anti-Goat IgG secondary antibodies; Related news: Ferroptosis/Oxytosis Antibody Panel is launched Therapeutic strategies against PDAC |
| Research Area | Ferroptosis/Oxytosis Study antibody |
| Calculated MW | 22 kDa |
Images (2) Click the Picture to Zoom In
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ARG64038 anti-GPX4 antibody WB image
Western Blot: Human Testis lysate (35 µg protein in RIPA buffer) stained with ARG64038 anti-GPX4 (Isoform a and c) antibody at 0.1 µg/ml dilution.
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ARG64038 anti-GPX4 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Testis. (Microwaved antigen retrieval with citrate buffer pH 6) stained with ARG64038 anti-GPX4 (Isoform a and c) antibody at 0.3 µg/ml dilution followed by HRP-staining.
