ARG41400
anti-GPX4 antibody
anti-GPX4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Ferroptosis/Oxytosis Study antibody
Publication8
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GPX4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GPX4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human GPX4. |
| Conjugation | Un-conjugated |
| Alternate Names | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; EC 1.11.1.12; MCSP; PHGPx; GSHPx-4; Glutathione peroxidase 4; GPx-4; snPHGPx; snGPx |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | ~ 20 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GPX4 |
| Gene Full Name | glutathione peroxidase 4 |
| Background | This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity). [UniProt] |
| Cellular Localization | Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30337 Ferroptosis / Oxytosis Antibody Panel Related products: GPX4 antibodies; GPX4 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Ferroptosis/Oxytosis Antibody Panel is launched Therapeutic strategies against PDAC |
| Research Area | Ferroptosis/Oxytosis Study antibody |
| Calculated MW | 22 kDa (mitochondrial) 20 kDa (cytoplasmic) |
Images (2) Click the Picture to Zoom In
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ARG41400 anti-GPX4 antibody WB image
Western blot:: Mouse Testis stained with ARG41400 anti-GPX4 antibody at 1:1000 dilution.
From Lipeng Li et al. Ecotoxicol Environ Saf (2023), doi: 10.1016/j.ecoenv.2023.115309, Fig. 2F1.
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ARG41400 anti-GPX4 antibody WB image
Western blot: 20 μg of Mouse testis and Rat testis lysates stained with ARG41400 anti-GPX4 antibody at 1:1000 dilution.
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Review for anti-GPX4 antibody
Application：WB
Sample：MCF7
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Inhibition of circRNA NGFR promotes ferroptosis in gallbladder carcinoma cells
WB / Human
Disrupting pro-survival and inflammatory pathways with dimethyl fumarate sensitizes chronic lymphocytic leukemia to cell death
WB / Human
Erythropoietin ameliorates cognitive dysfunction in mice with type 2 diabetes mellitus via inhibiting iron overload and ferroptosis
WB / Mouse
FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice
WB / Mouse
A novel quinoline derivative, DFIQ, sensitizes NSCLC cells to ferroptosis by promoting oxidative stress accompanied by autophagic dysfunction and mitochondrial damage
WB / Human
Blockage of Nrf2 and autophagy by L-selenocystine induces selective death in Nrf2-addicted colorectal cancer cells through p62-Keap-1-Nrf2 axis
WB / Human
Fermented and Aged Ginseng Sprouts ( Panax ginseng) and Their Main Component, Compound K, Alleviate Asthma Parameters in a Mouse Model of Allergic Asthma through Suppression of Inflammation, Apoptosis, ER Stress, and Ferroptosis
WB / Mouse
Oyster-Derived Tyr-Ala (YA) Peptide Prevents Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure by Suppressing Inflammatory, Apoptotic, Ferroptotic, and Pyroptotic Signals
WB / Mouse
