Overview

Product Description	Rabbit Polyclonal antibody recognizes GPX1
Tested Reactivity	Hu, Rat
Tested Application	ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	GPX1
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human GPX1.
Conjugation	Un-conjugated
Alternate Names	GPXD; Glutathione peroxidase 1; GPx-1; Cellular glutathione peroxidase; EC 1.11.1.9; GSHPX1; GSHPx-1

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size	22 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 24404 Rat GPX1 GeneID: 2876 Human GPX1 Swiss-port # P04041 Rat Glutathione peroxidase 1 Swiss-port # P07203 Human Glutathione peroxidase 1
Gene Symbol	GPX1
Gene Full Name	glutathione peroxidase 1
Background	This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function	Protects the hemoglobin in erythrocytes from oxidative breakdown. [UniProt]
Cellular Localization	Cytoplasm. [UniProt]
Calculated MW	22 kDa
PTM	During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine. [UniProt]

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